Ningbo Honor Imp & Exp Co.,Ltd , https://www.honormaket.com
Intelligent gene amplification instrument does not require enzymes per cycle
PCR technology, based on the principles of DNA replication, enables the amplification of specific genetic sequences. The double-stranded DNA from the Shanghai Xinzhuang Gene Amplifier can be separated into single strands through the action of various enzymes. When DNA polymerase and primers are introduced, two identical molecules of mussels—representing the target DNA—are replicated following the rules of base pairing. During a PCR experiment, it was observed that DNA denatures at high temperatures and can reanneal when cooled. This process allows for the controlled amplification of DNA by cycling through temperature changes, using designed primers to guide replication, along with DNA polymerase and dNTPs.
However, traditional DNA polymerases lose activity at high temperatures, requiring the addition of fresh enzyme in each cycle. This not only complicates the process but also increases costs, limiting the widespread use of PCR. A breakthrough came with the discovery of Taq DNA polymerase, a heat-resistant enzyme capable of withstanding temperatures above 90°C without losing function. This eliminated the need for adding new enzyme in every cycle, making PCR more efficient and cost-effective. As a result, PCR became widely applicable, especially in clinical settings.
The Shanghai Xinzhuang (LNB) gene amplification instrument is designed to perform PCR efficiently. It mimics the natural DNA replication process, relying on oligonucleotide primers that match both ends of the target sequence. The PCR process consists of three key steps: denaturation, annealing, and extension. First, the template DNA is heated to around 93°C, causing it to separate into single strands. Then, the temperature is lowered to approximately 55°C, allowing primers to bind to their complementary sequences. Finally, DNA polymerase extends the primers, synthesizing new DNA strands in a semi-conservative manner. This cycle repeats rapidly, typically taking 2–4 minutes per round, and within 2–3 hours, millions of copies of the target gene can be produced.
Shanghai Xinzhuang Instrument Co., Ltd. is a leading manufacturer of laboratory equipment, specializing in incubators, shaking incubators, and water bath shakers. With integrated R&D, production, sales, and service, the company provides essential tools for microbial, viral, bacterial, and cell culture studies, as well as biochemical reactions and genetic research across medical, agricultural, environmental, and scientific fields. Their PCR instruments are known for reliability, efficiency, and ease of use, supporting both research and practical applications in various industries.