Kyushu spatial analysis method for rapid detection of coliforms

The following is a rewritten and improved version of the original text in English, with added content to reach over 500 characters and presented as if written by a human. --- 1. **Scope of Application**: This method is ideal for the rapid detection of coliform bacteria in various food samples, raw ingredients, and purified water. It provides a quick and efficient alternative to traditional methods, suitable for both routine and on-site testing scenarios. 2. **Principle of the Method**: The test paper contains lactose, a color indicator, and a selective medium. After incubation, coliforms that ferment lactose produce acid, causing a color change from purple to yellow or red spots on a yellow background. This indicates a positive result for coliforms. The number of positive papers at each dilution is recorded, and the final count is determined using the MPN (Most Probable Number) table. 3. **Method Characteristics**: Compared to the traditional nine-tube method outlined in national standards, this technique simplifies the process into a single step, reducing the overall time from 78 hours to just 24 hours. It eliminates the need for preparing media, sterilizing equipment, and extensive cleaning. Additionally, it allows for immediate sampling without prior preparation, making it highly convenient for field use. 4. **Method of Operation** (1) **Sample Preparation**: Aseptically weigh 25 g (or 25 mL) of the sample into a container with 225 mL of sterile physiological saline. Shake thoroughly to create a 1:10 homogenate. Then, take 1 mL of this homogenate and transfer it to a test tube containing 9 mL of sterile saline to make a 1:100 dilution. Repeat the process for further dilutions, ensuring a new sterile pipette is used for each step. (2) **Inoculation**: Two dilutions are typically selected for testing. For most foods, 1:10 and 1:100 dilutions are used. For beverages and drinking water, the stock solution and a 1:10 dilution are recommended. Each dilution is inoculated onto three large paper discs (10 mL), six small discs (1 mL), and two small discs per bag are counted as one unit. Using a sterile pipette, 10 mL of the 1:10 dilution is added to a bag with large discs (equivalent to 1 g of sample). After soaking, lay the disc flat and repeat three times. Next, 1 mL of the same dilution is added to a bag with small discs (equivalent to 0.1 g of sample), also repeated three times. Finally, 1 mL of the 1:100 dilution is added to another set of small discs (equivalent to 0.01 g of sample), with three replicates. (3) **Incubation**: Place the inoculated paper discs in a 37°C incubator for 15–24 hours. The incubation time may vary slightly depending on the sample type and environmental conditions. 5. **Results Interpretation and Counting**: A positive result appears as a yellow color or red spots on a yellow background. A negative result remains purple or shows red spots on a purple-blue background, with no surrounding yellow. Record the number of positive papers at each dilution and refer to the MPN table to determine the coliform count. If the first dilution was the stock solution, divide the result by 10 accordingly. This ensures accurate quantification even when working with high-concentration samples. 6. **Notes**: If the sample has a pH below 7, adjust it to neutral using 1 mol/L sterile sodium hydroxide (NaOH) before testing. This prevents premature color changes due to acidic conditions, which could interfere with result interpretation. ![Kyushu spatial analysis method for rapid detection of coliforms](http://i.bosscdn.com/blog/20/14/12/235638734035.jpg) ![Kyushu spatial analysis method for rapid detection of coliforms](http://i.bosscdn.com/blog/20/14/12/235641658260.jpg) This method offers a practical, time-saving, and reliable approach to coliform detection, especially useful in food safety monitoring and quality control settings.

Curtain Rod Splice

In today's home decoration market, various types of Curtain Rod connection rods emerge endlessly, providing consumers with a wealth of choices. This article will introduce the different types of curtain rod connection rod and their characteristics in detail to help you better understand the market and choose the product that is right for you.

First, classification by material

1. Iron connection rod: This connection rod is usually made of cast iron or forged iron, which is durable and affordable. However, the iron connection rod may not be aesthetically pleasing and is prone to rust.
2. Stainless steel connecting rod: Stainless steel is a kind of corrosion-resistant and wear-resistant metal material, so stainless steel connecting rod is becoming more and more popular in the market. The stainless steel connection rod is beautiful in appearance, easy to clean, and has a long service life.
3. Aluminum alloy connecting rod: Aluminum alloy connecting rod has the characteristics of light, beautiful, easy to install, suitable for modern simple style of home decoration. However, aluminum alloys are not as strong and corrosion-resistant as stainless steel.

2. Classification by use

1. Fixed connection rod: Fixed connection rod is usually used to fix the curtain rod to the wall, suitable for places without Windows or where the curtain needs to be fixed.
2. Roller-type connecting rod: The roller-type connecting rod enables the curtain to slide easily through the pulley system, which is suitable for situations where the position of the curtain needs to be adjusted frequently.
3. Telescopic connecting rod: The telescopic connecting rod can be adjusted according to the size of the window, suitable for different sizes of Windows, saving space and easy to use.

3. Classification by style

1. Traditional style: Traditional style connecting rod is usually straight rod or curved shape, suitable for traditional or retro style home decoration.
2. Modern simple style: The modern simple style connection rod is usually designed for simple lines, which is suitable for modern or minimalist style home decoration.
3. Creative design style: The creative design style connection rod is more personalized, such as with flowers and birds, geometric patterns, etc., to add a touch of art to the home.

4. Other features

1. Material surface treatment: Many connecting rods are coated on the surface, such as galvanized, spray, etc., to improve rust prevention and aesthetic appearance.
2. Material color: In addition to the common white and black, there are now many other colors of the connection rod to choose from, such as gold, silver, gray, etc., to add a highlight to home decoration.
3. Installation mode: Some connecting rods support wall-mounted installation and floor installation to meet the needs of different scenarios.

Summary: When choosing a curtain rod connection rod, you need to choose the right type according to your home style, budget and usage needs. By understanding the various types and features, you can easily find the right product for you. Hopefully, this article will help you make an informed decision when choosing a curtain rod connection rod.

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