Alpha-amylase (Bacillus subtilis) / Medium-temperature amylase / liquefied amylase / liquefied enzyme / α-1,4 dextrinase / bacterial amylase / α-amylase / dextrinase

English name: α-Amylase (Bacilus subtilis); 1,4-α-D-Glucan-glucanohydrolase
Other names: Medium temperature amylase; liquefied amylase; liquefied enzyme; α-1,4 dextrinase; bacterial amylase; α-amylase; dextrinase
CAS number: 9000-90-2

Level: BR
Vitality: ≥4000U / g
Activity definition: 1ml of enzyme solution at 60 ℃, PH6.0, 1 hour of liquefied soluble starch 1g is an enzyme activity unit, expressed in u / ml

Moisture: ≤8%
Thermal stability: more stable below 60 ℃, the most suitable operating temperature is 60 ~ 70 ℃, and can be applied to the liquefaction process up to 90 ℃
PH stability: It is more stable from pH 6.0 to 7.0, the most suitable pH 6.0, the effect of severe inactivation below pH 5.0 The effect of calcium ion concentration on enzyme activity: calcium ion can improve the stability of enzyme activity, without calcium Ion, enzyme activity completely lost trait: yellow brown or off-white powder. Extracted by Bacillus subtilis, soluble in water. Under medium temperature conditions, this enzyme can rapidly hydrolyze the refined α-1,4 glucosidic bonds of starch molecules, arbitrarily cut the starch molecules from the inside of short-bonded dextrins and a small amount of low molecular sugars, amylose Both amylopectin and amylopectin are decomposed in an irregular form, so that the viscosity of starch slurry drops rapidly (that is, liquefaction, also known as liquefaction enzyme). When the storage temperature is not greater than 25 ℃, the enzyme activity should be no less than 90% of the marked enzyme activity within half a year; in order to improve the enzyme activity and stability, the calcium ion concentration should be not less than 50-70PPM; A certain surfactant has a certain effect on enzyme activity. When the liquefaction temperature is low or the material concentration is too high, the amount of enzyme used must be increased appropriately; when the pH of the material is greater than 8.0 or less than 5.0, it will cause loss of enzyme activity. Use: Biochemical research. Can quickly liquefy starch to generate low molecular weight.
Storage: 2 ~ 8 ℃

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