The main problem of serum medium
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The main problem of serum medium
1. There may be as many as hundreds of ingredients in serum, and its exact composition, content and mechanism of action are still unclear, especially for some of these peptide growth factors, hormones and lipids, which have not been fully understood, which gives research work Brings many difficulties.
2. Serums are produced in batches, and the batches vary greatly, and the storage period of the serum is at most one year. Therefore, it is extremely difficult to ensure the similarity of each batch of serum, which limits the standardization and continuity of the experiment.
3. For most cells, in the body state, the serum is not the physiological fluid they are in contact with, only the serum is contacted during the process of injury healing and blood clotting. Therefore, the use of serum may change the normal state of certain cells in the body, and the serum may promote certain The growth of cells (fibroblasts) simultaneously inhibits the growth of another type of cells (epidermal cells).
4. Serum contains substances that are toxic to cells, such as polyamine oxidase, and can react with polyamines from highly proliferating cells (such as spermine, spermidine) to form polyspermine with cytotoxic effects. Complements, antibodies, bacterial toxins, etc. can affect cell growth and even cause cell death.
5. Different animals have different origins and batch numbers. The quality of each batch is very different, and its composition cannot be kept the same.
6. The mycoplasma and virus may be brought into the material, which may have a potential impact on the cells, which may lead to experimental failure or unreliable experimental results.
7. In large-scale production, the source of serum is becoming more and more difficult and expensive, which constitutes one of the main parts of the production cost of animal cell culture.