Mouse dentin matrix protein 1 (DMP1) ELISA detection kit user's Guide Detection principle The kit uses double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with mouse dentin matrix protein 1 (DMP1) capture antibody, add specimens, standards, and HRP-labeled detection antibodies in sequence, incubate and wash thoroughly. The color is developed with the substrate TMB, which is converted into blue under the catalysis of peroxidase and into the final yellow under the action of acid. The color depth is positively correlated with mouse dentin matrix protein 1 (DMP1) in the sample. Measure the absorbance (OD value) at 450nm with a microplate reader to calculate the sample concentration. Sample collection, processing and storage methods 1. Serum: Use a test tube that does not contain pyrogens and endotoxins. Avoid any cell stimulation during the operation. After collecting blood, centrifuge at 3000 rpm for 10 minutes to quickly and carefully separate serum and red blood cells. 2. Plasma: EDTA, citrate or heparin anticoagulation. Take the supernatant by centrifugation at 3000 rpm for 30 minutes. 3. Cell supernatant: centrifuge at 3000 rpm for 10 minutes to remove particles and polymers. 4. Tissue homogenate: Add tissue to the right amount of saline and mash. Take the supernatant by centrifugation at 3000 rpm for 10 minutes. 5. Preservation: If the sample is not tested in time after collection, please aliquot it in one dose and freeze it at -20 ℃ to avoid repeated freezing and thawing. Thaw at room temperature and ensure that the sample is thawed evenly and fully. Bring your own items 1. Microplate reader (450nm) 2. High-precision sampler and tip: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL 3. 37 ℃ thermostat Operation notes 1. Store the kit at 2-8 ° C and equilibrate at room temperature for 20 minutes before use. The concentrated washing liquid taken out of the refrigerator will have crystals, which is a normal phenomenon. The water bath is heated to completely dissolve the crystals before use. 2. The slats not used in the experiment should be immediately returned to the ziplock bag, sealed (dry at low temperature) and stored. 3. The standard dilution can be regarded as a negative control or blank; the sample after pretreatment does not need to be diluted, just take 10μL and add it. 4. Perform the incubation operation strictly in accordance with the time, amount of liquid and sequence indicated in the manual. 5. Shake all liquid components thoroughly before use. Kit composition name 96-well configuration 48 hole configuration Remarks Microplate 12 holes × 8 12 holes × 4 strips no Standard (240pg / mL) 0.6mL 0.6mL Dilute according to the instructions Standard dilution 6mL 3mL no Sample diluent 6mL 3mL no Detection antibody-HRP 10mL 5mL no 20 × washing buffer 25mL 15mL Dilute according to the instructions Substrate A 6mL 3mL no Substrate B 6mL 3mL no Stop solution 6mL 3mL no Sealing film 2 sheets 2 sheets no Instructions 1 serving 1 serving no Ziplock bag 1 1 no Note: The standard product is diluted with standard product diluent in order: 240, 120, 60, 30, 15, 7.5pg / mL Reagent preparation Dilution of 20 × washing buffer: 1:20 dilution of distilled water, that is, 1 part of 20 × washing buffer plus 19 parts of distilled water. Washing method 1. Manually wash the plate: throw away all the liquid in the hole, fill each hole with the washing liquid, leave the liquid in the hole after standing for 1 min, pat dry on the absorbent paper, and wash the plate 5 times in this way. 2. Automatic plate washing machine: Inject 350μL of washing liquid into each well, soak for 1min, and wash the plate 5 times. Steps 1. Take out the required slats from the aluminum foil bag after equilibrating at room temperature for 20 min. The remaining slats are sealed with a ziplock bag and put back at 4 ° C. 2. Set up standard wells and sample wells, add 50μL of standard products of different concentrations to the standard wells; 3. Add 10μL of the sample to be tested first, and then add 40μL of the sample diluent; 4. Then add 100 μL of detection antibody labeled with horseradish peroxidase (HRP) to each well of the standard and sample wells, seal the reaction well with a sealing plate, and incubate for 60 min in a 37 ° C water bath or incubator. 5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution, let stand for 1min, shake off the washing solution, pat dry on the absorbent paper, and repeat washing the plate 5 times (you can also wash the plate with a washing machine) 6. Add 50 μL of substrate A and B to each well, and incubate at 37 ° C in the dark for 15 minutes. 7. Add 50μL of stop solution to each well, and measure the OD value of each well at 450nm wavelength within 15min. Result judgment Draw standard curve: In the Excel worksheet, the standard product concentration is used as the abscissa, and the corresponding OD value is used as the ordinate. The standard product linear regression curve is drawn, and the concentration value of each sample is calculated according to the curve equation. Kit performance 1. Accuracy: The correlation coefficient R between the linear regression of the standard product and the expected concentration is greater than or equal to 0.9900. 2. Sensitivity: The minimum detection concentration is less than 1.0 pg / mL. 3. Specificity: Does not cross-react with other soluble structural analogs. 4. Repeatability: The coefficients of variation within and between panels are less than 15%. 5. Storage: 2-8 ℃, protected from light and moisture. 6. Validity: 6 months Disclaimer 1. The kit is for research use only, and should not be used for clinical or human experiments, otherwise all the consequences will be borne by the experimenter, and the company will not be responsible. 2. Strictly follow the instructions, and the experimenter violates the instructions, and the consequences will be borne by the experimenter. Plush key chain its main materials are plush fabric and polypropylene cotton packing. There are four main types of plush fabrics. The first common yarn, made of BOA material, can be classified as light, usually straight, and can be divided into Yin and Yang yarns in different directions. And matte gauze namely matte color, basically do not have Yin and Yang two faces. The second type of yarn is called specialty yarn. It has sheeting and long and short wool. Wool length is controlled at 4-20 mm. The third kind is called plush, wool in 20-120mm range of growth, 20-45mm range can do any wool growth, 45mm above only 65mm and 120mm, belongs to long hair, wool is straight, not easy to curl. The fourth type is called curly hair, which is usually grainy hair, wool, or hair roots that are bunched and rolled up. Polypropylene fiber cotton, commonly known as baby cotton, hollow cotton, also known as cotton, chemical fiber man-made materials polypropylene fiber, polypropylene fiber (a plastic optical density, special attention to polypropylene fiber is different from polyester can not be confused) from the difference in the process: there are mainly hollow fiber and two kinds, respectively: good elasticity of the product, feel smooth. Our company produces plush key chain beautiful and generous, durable, three-dimensional strong, bright color, compact. Products can be made according to customer requirements of different specifications, color, thickness, grade, packaging, etc., LOGO can be customized, is a good choice for promotional gifts. Key chain not only can adorn your life, but also can highlight your noble, a person's key chain with what kind of gift can see this person's personality, life, and hobbies, also can see who can bring you good luck.
Major description:
Company products to environmental protection materials, exquisite technology, various supporting facilities, advanced technology, perfect management, excellent quality, reasonable price, rapid development, strict confidentiality, perfect after-sales service. Only detailed product drawings (or samples) are required to provide customers with one-stop service from development to after-sales service.
Plush Toy Key Chain,Custom Plush Toy Key Chain,Fox Doll Key Chain,Plush Stuffed Toy Key Chain SHEN ZHEN HAO XIAN LONG TECHNOLGY.CO.,LTD , https://www.haoxianlongtoys.com
1. Cloth ordering: in modern society, there is a clear division of labor, and manufacturers have their own responsibilities for the manufacturing of cloth and the production of doll products. Production problems tend to occur in non-factory processes. For this reason, it is particularly important for us to control the quality of materials. We strictly select several fixed material cooperative factories, which not only ensure the richness of material color, but also control the stability of material quality.
2. Making knife mold: the time required for making the knife mold of plush dolls is short, and it only takes a few hours from the start of making to the official use of the knife mold. Come on! Low price! Fashion! It has become an important factor for most companies to choose plush dolls as promotional gifts, conference gifts and event souvenirs.
3. Fabric opening: there are four ways to open the plush doll: punching machine, laser, electric shearing, and thermal shearing. Punch material opening needs to unfold the whole roll of cloth lamination first. The more layers there are, the lower the material opening cost will be. In the industry, generally, the 28 to 33 layers shall prevail. The factory will be subject to the 28 floor, in each process in strict accordance with the standard implementation to ensure the large cargo and sample effect of the anastomosis.
4. Electric embroidery and silk-screen printing: electric embroidery and silk-screen printing are the main presentation forms of logo or pattern on the customized figures; Silk-screen printing is a printing process, most of the cases are not necessarily electric embroidery looks high-grade. However, electrical embroidery cannot completely replace silk screen printing. For example, small and complex patterns or logo electrical embroidery cannot be realized. Only silk screen printing can show fine and clear patterns. For example, some parts of the requirements to make patterns at the same time to avoid the hardening of the parts affect the appearance (electrical embroidery will lead to the hardening of the parts), then also need to choose the process of electrical embroidery to show the logo or pattern. Of course, we don't have to worry about which process to choose to show our logo. We will provide professional solutions according to the actual situation.
5. Stitching and cutting: stitching is a process of stitching all cut pieces together, which is one of the most important processes in all processes. Whether the sewing process is strictly in accordance with the standardization will directly affect the smoothness of the appearance of the doll; Our factory has established strict sewing standards. A. Sewing seam 45 mm, uniform stop, needle spacing 12 inches. B. Fine needle with 11# needle and 402 fine thread. C. All point positions and bone positions must be aligned and shall not be exploded.
6, rolling cotton: cotton seems to be a simple process, in fact, is a very important process; Indeed, it is very easy to learn cotton, a new master as long as five minutes can learn to cotton. But it's not easy to do the simple job of making cotton well. Cotton is not only to cotton into the doll's shell inside, cotton also needs to be flushed evenly, to ensure that the finished product is a round full appearance. Simple things can be repeated to become an expert, to become a cotton expert needs at least three years to accumulate; And I plant all the cotton puncher is more than five or six years of experts.
7, sewing manual: sewing, manual, is the final process of plush toys finished products; Each doll in the cutting stitching stage will leave a position without stitching, the position is commonly known as leather or cotton left mouth position, as the name suggests is reserved for leather and cotton. And this position can only wait until the cotton is finished after the manual suture.
8. Product quality inspection: the product quality inspection is the last quality control link. After the product passes the quality inspection, it becomes the final product to be delivered to the customer. As a product quality inspector, I need not only the carefulness to find out product defects comprehensively, and the technology to repair defects, but more importantly, a sense of responsibility and a mindset that customers will not be satisfied if I am not satisfied.
