The concept and principle of ELISA

ELISA is short for enzyme-linked immunosorbent assay. It is an immunoenzyme technology developed after immunofluorescence and radioimmunoassay. This technology has developed very rapidly since its introduction in the early 1970s, and it has been widely used in many fields of biology and medical sciences.

The concept and principle of ELISA

ELISA is based on immunological reaction, a highly sensitive test technique that combines the specific reactions of antigen and antibody with the efficient catalytic effect of enzyme on substrate. Since the reaction of antigen and antibody is carried out in the well of a solid phase carrier-polystyrene microtiter plate, after each reagent is added to the incubation, excess free reactants can be removed by washing to ensure the specificity of the test results With stability. In practical applications, through different designs, there are many specific method steps. Namely: indirect method for detecting antibodies, double antibody sandwich method for detecting antigens, and antigen competition method for detecting small molecule antigens or haptens, etc. More commonly used are ELISA double antibody sandwich method and ELISA indirect method.

Shanghai Yanhui Biotechnology Co., Ltd. is one of the major domestic suppliers. The company acts as an agent for ELISA kits of different brands and price levels. Tens of thousands of antibody consumable reagents, etc., many varieties, good quality, high sensitivity, affordable, can provide free testing services (save you time)

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