Specifically how to use the UV detector

The UV detector is an ultraviolet device in liquid chromatography. It uses R212 photomultiplier tube, which has the advantages of stable performance, flexible use, high reliability and easy maintenance. How should the user use the UV detector? The following is a small series to introduce the use of the UV detector, I hope to help everyone.

How to use the UV detector

1. First connect the host and recorder two parts of the circuit, then plug the host's power plug into the 220V power socket.

2. The wavelength setting before delivery is 280nm. If other wavelengths are needed, just turn the wavelength conversion knob on the right side of the instrument to dial the desired file.

3. Press the power switch indicator of the main unit to light. If the transmittance is 100%T, turn the knob (ie sensitivity) to the T position. Adjust the light knob and the LED digital display also displays the number.

4. If the optical density A test is performed, turn the range knob (ie sensitivity) to the T position. Adjust the light amount knob so that the LED digital panel display is around 98. The instrument is preheated for about 1.5 hours. When the baseline is stable, the instrument is calibrated.

5. Connect the plastic tube at the lower end of the column to the plastic tube of the main unit, and send the plastic tube to a part of the collector or test tube. At this time, the eluent passes through the sample pool.

6. Adjust the light amount knob, set the range knob to 100% T file, make the LED digital display 100, T dial to A file, make the LED panel read 0 or so, if the transmittance T test, it can be in the column The sample was loaded and the sample was separated and analyzed.

7. If the optical density A test is performed, turn the range knob (ie sensitivity) to the desired optical density A, and adjust the light amount knob so that the LED panel is 0. At this point, the recorder returns to near zero (note: near zero, you can customize it in the experiment).

8. If the peak of the plot is too small, turn the range knob (ie sensitivity) to the desired position.

9. During the test, the “light quantity” no longer changes. The test is completed and the sample cell and tubing are rinsed with distilled water.

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