Specimen: serum or plasma Test principle: The EPO kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). Standards with known EPO concentrations and samples with unknown concentrations are added to microwell enzyme plates for detection. First, EPO and biotin-labeled antibodies are incubated simultaneously. After washing, HRP labeled with avidin was added. After incubation and washing, the unbound enzyme conjugate is removed, and then substrates A and B are added to act simultaneously with the enzyme conjugate. Produce colors. The color depth is proportional to the concentration of EPO in the sample. Bring your own material distilled water. Sampler: 5ul, 10ul, 50ul, 100ul, 200, 500ul, 1000ul. Oscillator and magnetic stirrer etc. Safety Avoid direct contact with the stop solution and substrates A and B. Once exposed to these liquids, rinse with water as soon as possible. Do not eat, drink, smoke or use cosmetics during the experiment. Do not use your mouth to absorb any ingredients in the kit. Handling precautions Reagents should be stored according to label instructions, and returned to room temperature before use. The sparse standards should be discarded and cannot be stored. The slats not used in the experiment should be immediately returned to the packaging bag, sealed and stored to avoid deterioration. Unused other reagents should be packed or covered. Do not mix reagents of different batches. Use before warranty. Use a disposable pipette tip to avoid cross-contamination, and avoid using a sampler with a metal part when drawing the stop solution and substrates A and B. Use a clean plastic container to prepare the washing solution. Mix all components and samples in the kit thoroughly before use. When washing the enzyme-labeled plate, it should be fully patted dry. Substrate A should evaporate to avoid opening the lid for a long time. Substrate B is sensitive to light and avoid prolonged exposure to light. Avoid contact with hands, it is toxic. The OD value should be read immediately after the experiment is completed. The order of adding reagents should be the same to ensure that all wells are incubated for the same time. Perform the incubation operation according to the time, amount and sequence of the instructions in the instructions. Sample collection, processing and storage of human erythropoietin ELISA test kit. Serum ---- avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins. After collecting blood, centrifuge at 1000 × g for 10 minutes to separate the serum and red blood cells quickly and carefully. Plasma ----- EDTA, citrate, heparin plasma can be used for detection. Centrifuge at 1000 × g for 30 minutes to remove particles. The cell supernatant was centrifuged at 1000 × g for 10 minutes to remove particles and polymers. Storage ------ If the sample is not used immediately, it should be divided into small parts and stored at -70 ℃ to avoid repeated freezing. If possible, do not use hemolysis or hyperlipidemia. If there are a large number of particles in the serum, centrifuge or filter before testing. Do not thaw at 37 ° C or higher. Thaw at room temperature and ensure that the sample is thawed evenly and adequately. Reagent preparation standards: Serial dilutions of standards should be prepared during the experiment and cannot be stored. Before dilution, the standard was mixed by shaking. Dilution of washing buffer (50 ×): 50-fold dilution with distilled water. Before use, mix all reagents thoroughly. Don't make the liquid generate a lot of foam, so as to avoid adding a large number of bubbles during sample addition, which will cause errors in sample addition. The number of slats required is determined by the number of samples to be tested plus the number of standard products. It is recommended to make multiple holes for each standard and blank hole. Each sample is determined according to its own quantity, and those that can use multiple holes can be used as much as possible. Add 50ul of the diluted standard to the reaction well and 50ul of the sample to be tested in the reaction well. Immediately add 50ul of biotin-labeled antibody. Cover the membrane plate, gently shake and mix, and incubate at 37 ° C for 1 hour. Shake off the liquid in the wells, fill each well with washing liquid, shake for 30 seconds, shake off the washing liquid, pat dry with absorbent paper. Repeat this operation 3 times. If washing with a plate washer, the number of washes is increased once. Add 80 ul of streptavidin-HRP to each well, gently shake and mix, and incubate at 37 ° C for 30 minutes. Shake off the liquid in the wells, fill each well with washing liquid, shake for 30 seconds, shake off the washing liquid, pat dry with absorbent paper. Repeat this operation 3 times. If washing with a plate washer, the number of washes is increased once. Add 50ul of substrate A and B to each well, mix gently by shaking, and incubate at 37 ° C for 10 minutes. Avoid light. Remove the enzyme labeling plate and quickly add 50ul of stop solution. After adding the stop solution, the results should be measured immediately. The OD value of each well was measured at a wavelength of 450 nm. The results of the human erythropoietin ELISA test kit limited to standard No. 6 and above are non-linear, and accurate results cannot be obtained based on this standard curve. Kit performance 1. Sensitivity: The minimum detection concentration is less than No. 1 standard. Linearity of dilution. The coefficient R of the linear regression of the sample and the expected concentration is 0.990. 2. Specificity: Does not react with other human cytokines. 3. Repeatability: The coefficients of variation within and between plates are less than 10%. Judgment and analysis of results 1. Instrument value: read the OD value of each well on a microplate reader with a wavelength of 450 nm. 2. Take the absorbance OD value as the ordinate (Y), and the corresponding EPO standard concentration as the abscissa (X) The corresponding curve is made, and the EPO content of the sample can be converted from the standard curve to the corresponding concentration according to its OD value. 3. Detection value range: 0-8.0IU / ml 4. Sensitivity: 0.01 IU / ml human erythropoietin ELISA detection kit DA1914-0.2ml glypican 1 Phosphatidyl proteoglycan-1 antibody 0.2ml DA1915-0.1ml glypican 3 / GPC3 (Intestinal protein OCI-5; GTR2-2; MXR7) Phosphatidyl proteoglycan-3 antibody 0.1ml DAP034-0.1ml phospho-ATR / ACTR (Ser428) Phospho-ATR antibody 0.1ml DAP035-0.1ml Phospho- ATP1A1 (Ser23)? Sodium potassium phosphate ATPase protein a1 antibody 0.1ml DAP036-0.1ml phospho-ATP citrate lyase (Ser455)? Phosphophosphoryl adenocitrate citrate lyase antibody 0.1ml DAP037-0.1ml phospho-BAD (Ser128)? Phosphorylation-related death-promoting factor antibody 0.1ml DA1915-0.2ml glypican 3 / GPC3 (Intestinal protein OCI-5; GTR2-2; MXR7) Phosphatidyl proteoglycan-3 antibody 0.2ml DA1916-0.2ml Glypican 4 (Glypican proteoglycan 4) Phosphatidyl proteoglycan-4 antibody 0.2ml DA1918-0.2ml Grb2 / Ash (Growth factor receptor-bound protein 2) growth factor receptor binding protein 2 antibody 0.2ml Carton Erector refers to automatically complete unpacking, forming, and bottom folding. And now complete the next part of the adhesive tape, open the box board stacked into cardboard, the bottom of the box is folded according to a certain procedure, and sealed with tape and then delivered to the special equipment of the boxing machine. Automatic carton forming machine and automatic unpacking machine are the assembly line equipment for automatic unpacking of large batches of cartons, automatic folding of the lower cover and automatic sealing of the bottom tape. The machine is controlled by PLC+ display screen, which is greatly convenient for operation and is essential for automated large-scale production. Case Erector,Carton Erector,Case Erector Machine,Carton Erector Machine Jining Myway Machinery Co., Ltd. , https://www.mwpackall.com