In order to compare the detection effect of three anti-M. Bovis (M. bovis) serum antibodies with ELISA kits, the ELISA kit used three kinds of M. bovis serum antibody ELISA diagnostic kits for 38 positive samples (19 natural infections, 19 18 artificial infections) and 37 negative samples were tested. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and compared with the CUTOFF value, so as to determine the presence or absence of Mycoplasma bovis in the specimen. ELISA kit In addition, the detection results of bovine infectious pleura pneumonia international standard serum (PS2) by three kits showed that both HVRI kit and Kit 1 were negative, and Kit 2 was positive. Therefore, the HVRI kit and Kit 1 are more suitable for M. bovis detection and epidemiological investigation. The microbore plate is coated with purified bovine Mycoplasma antibody to make a solid phase antibody, which can be combined with Mycoplasma in the sample, washed to remove unbound antibody and other components, and then combined with HRP-labeled Mycoplasma antibody to form an antibody-antigen -Enzyme-labeled antibody complex, after thorough washing, the substrate TMB is added for color development. The Mycoplasma bovis enzyme-linked immunoassay kit uses double-antibody sandwich enzyme-linked immunoassay (ELISA) to determine Mycoplasma bovis in the specimen. Consistency maintenance results show that the consistency of HVRI kit and Kit 1 is high; Kit 2 and HVRI kit, Kit 2 and Kit 1 have moderate consistency. TMB is converted into blue under the catalysis of HRP enzyme, and into the ultimate yellow under the action of acid. The results of the ELISA kit showed that the detection results and comprehensive test results of the HVRI kit prepared by this laboratory and the commercial kit Kit 1 reached 92% and 96%, respectively; while the commercial kit Kit 2 test results and comprehensive The coincidence rate of the test results is only 74.67%.
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Comparison of ELISA kits for detection of antibodies against Mycoplasma bovis
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