Our Pet Dog Clothes producing working-shop was established in 2006. We currently employ 300 skilled workers and 25 administrative personnel. Our dog costumes are manufactured according to EN71/ASTM stipulations. Presently, our dog costumes exports are valued at more than USD $3,000,000.00 every year, and main export markets include European country & USA. Some of our management have had over 12 years of experience in pet supplies industry, And have rich experience in working and cooperating with customers, Our business team provide highly efficient customer services and replies to your inquiries promptly. Quality products, punctual delivery and superior services are all included in our basic service to our clients! Please contact us for further details and we will promptly answer any questions you may have. Pet Dog Clothes,Dog Clothes,Pet Clothes,Designer Dog Clothes Ningbo Movepeak Pet Supplies Co.,LTD. , https://www.pet-supplies-factory.com
We also accept Pet Dog Clothes OEM & ODM orders,That means we can custom the Pet Dog Costumes & Dog Clothes totally according to your design,logo and color. We sincerely hope to establish long-term business relationship and cooperate with all the friends at home and broad.We trust Premium Quality Made Everything Easy!
Method for extracting DNA from blood clot
Operation steps: 1. Take 15ml grinder, add 5ml blood clot, 4-5ml hemolysis reagent, grind until no clot is present. Transfer to a 50 ml centrifuge tube at 2500 rpm for 10 min. 2. Go to the supernatant, wash the fake hemolysis reagent once, 2500 rpm, 10 min, until no red is present. 3. Remove the supernatant, add 1 ml of cell lysate, transfer to a centrifuge tube, add 20 mg/ml proteinase K 10 ml, and water bath at 56 ° C for 3 hours. 4. Add 1 ml of balanced phenol to each tube and mix at 6000 rpm for 10 min. 5. Repeat step 4. 6. Take the supernatant in a 2 ml EP tube, add an equal volume of chloroform / isopropanol, mix, 6000 rpm, 10 min. 7. Take the supernatant in another 2 ml EP tube, add NaAc, mix, add an equal volume of isopropanol, mix, and observe a white precipitate, 10000 rpm, 5 min. 8. Remove the supernatant, add 0.3 ml of absolute ethanol, 10000 rpm, 5 min, remove alcohol, and air dry. 9. Add TE solution, water bath until DNA is dissolved, and measure DNA concentration. Matters needing attention: 1. Observe the color of phenol, usually yellow. If it turns pink, it means it is oxidized and can not be used again. 2. Phenol is highly corrosive and easily causes burns. Once the skin is contaminated, rinse with water and wash with soap or diluted soda. Remember to use alcohol instead. 3. Hemolysis reagent, cell lysate, TE solution requires high temperature and high pressure disinfection. 4. The nucleic acid absorbs ultraviolet light by containing a conjugated benzene ring, and the maximum ultraviolet light absorption wavelength of DNA and RNA is 260 nm. When the light absorption value of 5.260 nm is 1 (A260 = 1) and the mass concentration of dsDNA is equivalent to 50 ug/ml, the concentration of dsDNA is: A260 value × 50 ug / ml × dilution factor. 6. Purity: A260 /A280 indicates that if the value of pure dsDNA is about 1.8, if it is greater than 1.8, it indicates RNA contamination. If it is less than 1.8, it indicates that there is protein or saturated phenol contamination in the sample. 7. The extracted DNA should be completely dissolved before measuring its concentration, and when measuring the concentration, the diluted sample must be mixed, otherwise the measured results will have a large deviation.