Enzyme activity unit

Enzyme activity unit

Enzyme activity unit (U, active unit)

Unit of measure for enzyme activity.

The 1961 International Enzyme Conference stipulated:

1 unit of enzyme activity refers to the amount of enzyme that can convert 1 micromole of substrate in 1 minute under certain conditions (25 ℃, others are the most suitable conditions), or 1 micromole of related groups in the conversion substrate The amount of enzyme.

Enzyme activity unit: a unit used to express the size of enzyme activity, usually expressed in terms of enzyme amount.

One of them is called the International Unit of Enzyme Activity, which is defined as: the amount of enzyme required to convert 1 micromolar substrate or 1 micromolar related group in a substrate under specific conditions in 1 minute is called an International Unit (IU, Also known as U).

Another unit of enzyme activity specified by the International Conference of Enzymology is Kat, which is defined as the amount of enzyme that can convert 1 mole of substrate in 1 second under optimal conditions.

Conversion relationship between Kat and U: 1 Kat = 6 × 107U, 1U = 16.67n Kat enzyme specific activity (specific activity): refers to the catalytic activity of a certain enzyme contained in each milligram of protein. It is used to measure the purity of enzymes. It is the basic data frequently used in production and enzymology research.

Enzyme conversion number (Kcat): the number of substrate molecules that can be converted by each active center or per molecule of enzyme in a unit of time. Not commonly used in production.

Method for measuring enzyme activity:

End point method, dynamic method, UV-visible spectrophotometry, fluorescence spectrophotometry, enzymatic coupling, electrochemical method

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